Molecular Beta-lactamase Detection in GNB

“It’s use in clinical practice may contribute to better knowledge of the local epidemiology of Beta-lactam resistance genes and to more appropriate use of antimicrobial agents, reduction of costs, and improved patient outcome.”

Naaas et. al. j Clin Microbiol. 2011 Apr;49(4):1608-13.
  • Microarrays for Carbapenemases, ESBLs, and AmpCs
    • Improve your epidemiological insight
    • Investigate suspected multidrug resistance
    • Exclude outbreaks

[lead]Molecular detection of Beta-lactamase in Gram negative bacilli[/lead]

  • Carbapenemases
    • KPC, NDM, VM, IMP, OXA-48 LIKE, GES, GIM, SPM, OXA-23 LIKE, OXA-24 LIKE, OXA-58 LIKE
  • CTX-M ESBLs
    • CTX-M-1 group, CTX-M-1-like, CTX-M-15-like, CTX-M-3-like
    • CTX-M-32-like, CTX-M-2 group, CTX-M-8 & -25 group, CTX M-9 group
  • TEM ESBLs vs. non-ESBL
    • TEM wt, TEM E104k, TEM R164S, TEM R164C, TEM R164H, TEM G238S
    • SHV ESBLs vs non-ESBL
    • SHV wt, SHV G238S, SHV G238A, SHV E240K
  • Other ESBLs
    • VEB, PER, BEL, GES
  • AmpCs
    • CMY I/MOX, ACC, DHA, ACT/MIR, CMY ll, FOX